ABSTRACT

Šề tąi: Ứng dụng kỹ thuật chiếu xạ šể nāng cao hiệu suất cắt mạch chitin tạo Glucosamine dạng clo vą sunphat.
Chủ nhiệm: CN. Nguyễn Tấn Mān

 

Glucosamine hydrochloride is an amino sugar which is incorporated into the structure of body tissues. It comprises about 80% glucosamine, a compound helpful in maintaining joint health in individuals suffering from degenerative conditions such as arthritis. When orally ingested, it is selectively taken up by joint tissues to exert beneficial effects. Glucosamine may also have other therapeutic effects such as antiviral, anti-cancer, anti-aging, immune boosting or cholesterol lowering activity.

Glucosamine may be obtained by hydrolysis and deacetylation of chitin, a polymer of N-acetyl glucosamine  with hydrochloric acid.

In this work we prepare glucosamine hydrochloride and glucosamine sulfate from irradiated chitin in order to produce high-purity product with good yield.

The method comprising the steps of:

1.      Grinding the chitin

2.      Irradiation the chitin using gamma Co-60 source at 30 kGy.

3.      Digesting the chitin with prewarmed, concentrated HCl, by mixing the chitin with the HCl, and heating to 95oC for 2hrs to produce a slurry.

4.      Cooling the slurry to room temperature and filtering the precipitate

5.      Dissolving the precipitate in hot water with activated charcoal at room temperature.

6.      Filtering the solution and discarding the solids.

7.      Evaporating the solution to recover glucosamine solids.

8.      Washing the glucosamine solids with ethanol

9.      Drying the glucosamine solids.

Glucosamine sulfate is very hygroscopic and degrades rapidly when exposed to moisture. To avoid this problem, glucosamine sulfate is made from glucosamine hydrochloride by adding potassium sulfate and co-crystallizing the resulting mixture.

The method comprising the steps of:

1.      Dissolving 25.9g of  glucosamine hydrochloride in 84g of distilled water with stirring.

2.      Adding 10.6g of potassium sulfate and stirring was continued for about one hour at temperature of from 35oC to 45oC to complete the reaction.

3.      Precipitating the stable crystalline form by addition of a liquid precipitating agent which is miscible with water and in which the crystalline form has a solubility no greater than 0.1% (w/v), with stirring or evaporation under vacuum.

4.      Completing the precipitating by reducing the temperature of the mixture.

5.      Recovering the precipitated crystalline form.

The liquid precipiting agent is selected from the group consisting of acetone, ethanol, isopropanol… and the liquid precipiting agent is added in a proportion of from 4 to 5 parts by volume relative to the volume of aqueous solvent, over the period of from 3 to 4 hours. The stable crystalline form recovered is dried at a temperature of from 45oC to 60oC.